most recent 30 from http://asksci.com 2010-03-11T02:50:49Z http://asksci.com/feeds http://www.creativecommons.org/licenses/by-nc/2.5/rdf http://asksci.com/questions/166/how-to-do-in-vivo-imaging-with-mouse glucose 2010-03-10T02:07:57Z 2010-03-10T02:07:57Z

<p>I have read many papers about in vivo imaging with flies or fish. Is there any good set up to do in vivo imaging with mouse?</p>

http://asksci.com/questions/165/can-we-use-adpative-optics-in-microscopes glucose 2010-03-08T15:06:59Z 2010-03-08T15:06:59Z

<p>I have read how using adaptive optics help NASA getting better images with their telescopes. Just wondering can we use the same technique to improve our microscopes?</p>

http://asksci.com/questions/164/how-neutron-stars-die RocketSurgeon 2010-03-06T18:09:21Z 2010-03-06T18:09:21Z

<p>There are lot of popular sources explaining how neutron stars are born, but I could not find any theory explaining death of neutron stars. What process make them burn ? Do they burn and radiate ? What happens at the end of burn process ? And after the end ?</p> <p>Thank you</p>

http://asksci.com/questions/33/what-happend-at-icsb-2009-stanford Peter 2009-10-10T23:04:36Z 2010-03-06T01:24:07Z

<p>Did any one attend this conference?</p>

http://asksci.com/questions/152/how-does-a-confocal-microscope-work glucose 2010-02-20T08:40:02Z 2010-03-05T23:40:53Z

<p>Are there any easy to read articles or movies?</p>

http://asksci.com/questions/132/fluorescent-polarization-tag-to-avoid-background-fluorescence drummo2a 2010-01-22T16:00:00Z 2010-03-05T21:23:51Z

<p>I am trying to use fluorescence polarization to measure the size of complexes in serum and/or plasma. Does anyone know of a good fluor to use that will get around the problem of serum autofluorescence?</p>

http://asksci.com/questions/162/what-is-a260-value glucose 2010-03-05T08:13:38Z 2010-03-05T08:13:38Z

<p>What is a260 value?</p>

http://asksci.com/questions/160/molecular-cloning-guide glucose 2010-03-04T22:22:36Z 2010-03-05T03:45:05Z

<p>Is there any good guide for doing the first molecular cloning experiment?</p>

http://asksci.com/questions/48/what-is-the-t1-2-of-gus-gfp-rfp-and-yfp-rna-and-proteins noam chehanovsky 2009-10-15T08:33:34Z 2010-03-05T02:23:30Z

<p>what is the T1/2 of GUS, GFP, RFP and YFP, RNA and proteins?</p>

http://asksci.com/questions/159/what-is-a-pentane Philly Finest 2010-03-04T17:49:51Z 2010-03-04T20:16:12Z

<p>How much energy would be released for 3.5 moles of pentane. How much energy would be released for 10 moles of pentane. How much energy would be released for 0.75 moles of pentane.</p>

http://asksci.com/questions/158/what-is-nanodrop glucose 2010-03-03T18:46:33Z 2010-03-03T18:46:33Z

<p>Is there any clear definition about what is a nanodrop and what are the major advantages of nanodrop over a spectrophotometer ?</p>

http://asksci.com/questions/88/high-background-in-western-blot-what-should-i-do unknown (google) 2009-10-27T00:55:45Z 2010-03-03T12:26:01Z

<p>High Background in western blot, what should I do?</p>

http://asksci.com/questions/91/patchy-uneven-spots-all-over-the-blot-in-western-blot-what-should-i-do unknown (google) 2009-10-27T00:57:13Z 2010-03-03T02:23:12Z

<p>Patchy uneven spots all over the blot in western blot, what should I do?</p>

http://asksci.com/questions/87/extra-bands-in-western-blot-what-should-i-do unknown (google) 2009-10-27T00:55:13Z 2010-03-03T01:23:22Z

<p>Extra Bands in western blot, what should I do?</p>

http://asksci.com/questions/90/white-bands-ecl-method-in-western-blot-what-should-i-do unknown (google) 2009-10-27T00:56:43Z 2010-03-03T01:23:22Z

<p>White Bands (ECL method) in western blot, what should I do?</p>

http://asksci.com/questions/142/i-want-to-know-what-happens-to-tryptophan-absorption-of-light-light Aya 2010-02-02T11:02:58Z 2010-03-02T20:23:33Z

<p>what happens if the environment around the tryptophan changes from Hydrophobic to hydrophilic ? I know that in fluorescence there is a red shift , but I don't know what happens in absorption. is it the same? </p>

http://asksci.com/questions/39/how-do-i-know-the-spectra-for-a-particular-flurophore Peter 2009-10-12T20:56:13Z 2010-03-02T04:22:59Z

<p>Is there a complete database for flurohores?</p>

http://asksci.com/questions/104/what-are-the-standad-test-when-you-want-to-check-protein-function Aya 2009-11-05T18:00:53Z 2010-02-28T09:23:41Z

<p>I did some test to find out the function of a protein group. I would like to know of there are standard Test I can do, maybe something with a wider range of possibilities then what I am doing now.</p>

http://asksci.com/questions/154/calculation-for-elisa bioviski 2010-02-21T08:10:02Z 2010-02-24T19:40:14Z

<p>Hi, please tell me the methods for how to calculate the calculation regarding the ELISA. i ma new to this method since i have started to work out the calculations iam not able to work it out. please tell me any link or tutorials for the same. </p>

http://asksci.com/questions/66/atoms-in-the-observable-universe Jonathan Sampson 2009-10-19T18:24:27Z 2010-02-23T04:23:30Z

<p>It is estimated that there are 10⁸⁰ atoms in the observable Universe. What methods exist to reach this number, and with what degree of accuracy should they be treated?</p>

http://asksci.com/questions/156/bachelor-degree-means pradeep 2010-02-23T00:13:31Z 2010-02-23T00:13:31Z

<p>What do "bachelor" stands for in degree.Is it related to marital status?If so many married people study bachelor's degree.How?</p>

http://asksci.com/questions/153/what-is-fluorescence Ken 2010-02-20T08:41:25Z 2010-02-22T18:46:48Z

<p>What does that mean?</p>

http://asksci.com/questions/151/which-is-the-best-platform-solexa-solid-and-454 glucose 2010-02-20T08:34:45Z 2010-02-20T08:34:45Z

<p>i would know which is for you the best platform between Solexa, Solid and 454?and why?</p> <p>I am new about next generation sequencing and i would know why a lot of people suggest to use Illumina.</p> <p>Thank you </p>

http://asksci.com/questions/149/what-function-describes-the-temperature-in-the-surroundings-of-a-point-heat-sourc Jader Dias 2010-02-15T15:20:01Z 2010-02-16T23:35:47Z

<p>If you have a system with equally distributed temperature, and in the center of it is inserted a point source of heat (a point hotter than the rest of the system) after a given amount of time this heat is dispersed through the system in a way that the points closer to the center are hotter than the ones farther from it.</p> <p>What is the function of the distance that describes more precisely the temperature distribution in this system?</p>

http://asksci.com/questions/147/what-function-describes-the-heat-dispersion-more-precisely Jader Dias 2010-02-14T22:35:15Z 2010-02-15T00:27:57Z

<p>I am developing a heat map image generator and I have come to 2 solutions to calculate the intensity in a given point within a distance from the heat source:</p> <p>The first one is similar to wave dispersion:</p> <blockquote> <p>intensity = 1 / math.pow(1 + distance, 2)</p> </blockquote> <p>The second one looks nice on the screen, but I invented it myself</p> <blockquote> <p>intensity = math.pow(2, -distance)</p> </blockquote> <p>I don't know if any of the solutions above are near the real world behavior.</p>

http://asksci.com/questions/76/how-to-use-imagej Peter 2009-10-27T00:22:59Z 2010-02-04T09:23:16Z

<p>What are the best websites over there to teach how to use ImageJ?</p>

http://asksci.com/questions/146/has-anyone-watched-the-naturally-obsessed-movie-a-documentary-about-graduate-stu glucose 2010-02-04T01:30:13Z 2010-02-04T01:30:13Z

<p>It was a really good one</p> <p>Check it out.</p> <p>Good for desperate graduate students ...</p> <p><a href="http://www.naturallyobsessed.com/" rel="nofollow">http://www.naturallyobsessed.com/</a></p>

http://asksci.com/questions/145/primary-smooth-muscle-cell-culture Kyle Legate 2010-02-03T14:22:59Z 2010-02-03T14:22:59Z

<p>I am trying to do some functional assays on primary smooth muscle cells from young mice. When I say young I mean newborn esophagus or 3 week old aortic smooth muscle. There are not many cells for functional assays so I take them into cell culture for a while until they grow confluent, and then I try to stimulate them with an agonist (carbachol) and visualize contraction under the microscope. The problem appears to be that these cells lose their contractility really fast and I can't see any response to agonist, although smooth muscle actin expression is still quite high (so they are still 'smooth muscle cells'). I culture them in DMEM + 10% FBS on plastic. I read that primary SMCs will rexpress contractility makers if starved for 3 days, but this hasn't helped my situation--maybe this trick is human-specific?</p> <p>Does anyone have any tips on how I can keep these cells contraction-competent in culture?</p>

http://asksci.com/questions/99/what-is-the-best-nuclear-protein-loading-control Peter 2009-10-27T01:14:28Z 2010-02-03T11:24:25Z

<p>what is the best nuclear protein loading control?</p>

http://asksci.com/questions/86/faint-bands-weak-signal-in-western-blot-what-should-i-do unknown (google) 2009-10-27T00:54:47Z 2010-02-02T13:24:04Z

<p>Faint Bands (Weak Signal) in western blot, what should I do?</p>