What are the standad test when you want to check protein function? - Ask Science Questions most recent 30 from http://asksci.com2010-07-29T19:24:36Zhttp://asksci.com/feeds/question/104http://www.creativecommons.org/licenses/by-nc/2.5/rdfhttp://asksci.com/questions/104/what-are-the-standad-test-when-you-want-to-check-protein-functionWhat are the standad test when you want to check protein function?Aya2009-11-05T18:00:53Z2010-07-18T22:11:18Z
<p>I did some test to find out the function of a protein group. I would like to know of there are standard Test I can do, maybe something with a wider range of possibilities then what I am doing now.</p>
http://asksci.com/questions/104/what-are-the-standad-test-when-you-want-to-check-protein-function/105#105Answer by glucose for What are the standad test when you want to check protein function?glucose2009-11-08T02:38:12Z2009-11-08T02:38:12Z<p>Should you first check the sub cellular localization?</p>
http://asksci.com/questions/104/what-are-the-standad-test-when-you-want-to-check-protein-function/297#297Answer by derfritz for What are the standad test when you want to check protein function?derfritz2010-07-18T22:11:18Z2010-07-18T22:11:18Z<ol>
<li>Check the sequence in databases to get some ideas. Soluble or membrane? Any localisation signal?</li>
<li>Overexpress maybe as GFP-Fusionprotein and test your theory from (1)</li>
<li>Downregulate by RNAi and test your theory from (1) and (2)</li>
<li>Pull-down assay and check associated proteins</li>
</ol>