Fluorescent Polarization tag to avoid background fluorescence - Ask Science Questions most recent 30 from http://asksci.com2010-07-29T19:13:28Zhttp://asksci.com/feeds/question/132http://www.creativecommons.org/licenses/by-nc/2.5/rdfhttp://asksci.com/questions/132/fluorescent-polarization-tag-to-avoid-background-fluorescenceFluorescent Polarization tag to avoid background fluorescencedrummo2a2010-01-22T16:00:00Z2010-07-24T03:22:36Z
<p>I am trying to use fluorescence polarization to measure the size of complexes in serum and/or plasma. Does anyone know of a good fluor to use that will get around the problem of serum autofluorescence?</p>
http://asksci.com/questions/132/fluorescent-polarization-tag-to-avoid-background-fluorescence/134#134Answer by glucose for Fluorescent Polarization tag to avoid background fluorescenceglucose2010-01-22T19:30:43Z2010-01-22T19:30:43Z<p>First, can you measure the excitation and emission spectra for the serum you are interested in?</p>
<p>Second, I assume you want to compare the serum spectra to the spectra of known probes, which can be found here:</p>
<p><a href="http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html" rel="nofollow">http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html</a></p>