most recent 30 from http://asksci.com 2010-07-29T19:21:35Z http://asksci.com/feeds/question/85 http://www.creativecommons.org/licenses/by-nc/2.5/rdf http://asksci.com/questions/85/no-bands-observed-in-western-blot-what-should-i-do unknown (google) 2009-10-27T00:54:07Z 2010-02-02T11:21:29Z

<p>No Bands Observed in western blot, what should I do?</p>

http://asksci.com/questions/85/no-bands-observed-in-western-blot-what-should-i-do/92#92 unknown (google) 2009-10-27T00:58:43Z 2009-10-27T00:58:43Z

<p>Insufficient antibody</p> <p>Antibody may have low affinity to protein of interest. Increase antibody concentration (2-4 fold higher than recommended starting concentration). Antibody may have lost activity. Perform a Dot Blot. </p>

http://asksci.com/questions/85/no-bands-observed-in-western-blot-what-should-i-do/143#143 Kyle Legate 2010-02-02T11:21:29Z 2010-02-02T11:21:29Z

<p>Too many possibilities to make a suggestion.</p> <ol> <li>Is there protein on your blot? Maybe you transferred in the wrong direction.</li> <li>Did you add secondary antibody?</li> <li>Is you primary antibody still functioning?</li> <li>Did you expose long enough?</li> <li>Did you block with too much milk/BSA/whatever? Low abundance proteins can be covered.</li> <li>Did you load enough protein?</li> </ol>