most recent 30 from http://asksci.com 2010-07-29T19:24:44Z http://asksci.com/feeds/question/86 http://www.creativecommons.org/licenses/by-nc/2.5/rdf http://asksci.com/questions/86/faint-bands-weak-signal-in-western-blot-what-should-i-do unknown (google) 2009-10-27T00:54:47Z 2010-02-02T13:24:04Z

<p>Faint Bands (Weak Signal) in western blot, what should I do?</p>

http://asksci.com/questions/86/faint-bands-weak-signal-in-western-blot-what-should-i-do/98#98 unknown (google) 2009-10-27T01:02:53Z 2009-10-27T01:02:53Z

<p>Low protein-antibody binding</p> <p>Reduce the number of washes to minimum. </p> <p>Reduce NaCl concentration in Blotting Buffer used for wash steps (recommended range 0.15M - 0.5M). </p> <p>Reduce NaCl concentration in Antibody Solution (recommended range 0.15M - 0.5M). </p>