most recent 30 from http://asksci.com 2010-07-29T19:16:45Z http://asksci.com/feeds/user/3 http://www.creativecommons.org/licenses/by-nc/2.5/rdf http://asksci.com/questions/268/is-pcdna3-1-a-double-stranded-vector glucose 2010-04-21T07:05:47Z 2010-07-28T11:22:30Z

<p>is pcdna3.1 a double-stranded vector?</p>

http://asksci.com/questions/258/what-is-photomanipulation glucose 2010-04-20T21:34:51Z 2010-07-28T04:22:35Z

<p>What is photomanipulation?</p>

http://asksci.com/questions/260/pratical-tips-for-frap glucose 2010-04-20T21:38:14Z 2010-07-28T04:22:35Z

<p>Pratical tips for FRAP</p>

http://asksci.com/questions/264/what-is-flip-and-how-is-it-different-from-frap glucose 2010-04-20T21:43:52Z 2010-07-28T03:22:31Z

<p>What is FLIP and how is it different from FRAP?</p>

http://asksci.com/questions/175/what-is-pcdna3-t-vector glucose 2010-03-23T08:01:37Z 2010-07-28T02:22:30Z

<p>what is pcdna3 t vector</p>

http://asksci.com/questions/222/what-is-functional-genomics glucose 2010-04-06T19:55:15Z 2010-07-28T02:22:30Z

<p>What is functional genomics? </p>

http://asksci.com/questions/255/what-is-zeiss-on-your-campus glucose 2010-04-20T20:18:27Z 2010-07-28T01:22:30Z

<p>What is zeiss on your campus?</p>

http://asksci.com/questions/262/what-is-the-fist-paper-that-described-frap glucose 2010-04-20T21:41:15Z 2010-07-28T01:22:30Z

<p>What is the fist paper that described FRAP?</p>

http://asksci.com/questions/174/what-is-ha-in-science glucose 2010-03-23T08:00:52Z 2010-07-28T00:22:30Z

<p>What could be ha in science?</p>

http://asksci.com/questions/240/what-is-the-impact-of-coverslip-thickness-on-image-quality glucose 2010-04-14T18:25:34Z 2010-07-25T01:22:30Z

<p>What is the impact of coverslip thickness on image quality</p>

http://asksci.com/questions/243/double-digest-suggestions glucose 2010-04-17T18:55:26Z 2010-07-25T00:22:34Z

<p>double digest suggestions?</p>

http://asksci.com/questions/242/how-to-read-confocal-stacks-in-imagej glucose 2010-04-17T18:54:17Z 2010-07-24T23:22:34Z

<p>How to read confocal stacks in imagej</p>

http://asksci.com/questions/250/tuj1-staining-in-tissue glucose 2010-04-17T20:16:57Z 2010-07-24T23:22:34Z

<p>anyone has good experience with Tuj1 staining, it works for us but with pretty background, would love to work out a better protocol for one study... Any tips/informations would be highly appreciated</p>

http://asksci.com/questions/215/when-calculating-auto-correlation-function-for-fcs-why-are-the-results-different glucose 2010-04-02T02:16:10Z 2010-07-24T11:22:37Z

<p>Fluorescence correlation spectroscopy (FCS) is a common technique used by physicists, chemists, and biologists to experimentally characterize the dynamics of fluorescent species.</p> <p>The key of the technique is the auto correlation function. The (temporal) autocorrelation function is the correlation of a time series with itself shifted by time τ, as a function of τ:</p> <p>The formula is given by <img src="http://upload.wikimedia.org/math/9/8/d/98dee7cc43c03ceb17d86bdd45b86a8c.png" alt="G()=I(t)2I(t)I(t+)=I(t)2I(t)I(t+)−1"></p> <p>where <img src="http://upload.wikimedia.org/math/6/1/1/611fccc6453b32e1d4588ba2d09812e5.png" alt="I(t)=I(t)−I(t)"> is the deviation from the mean intensity.</p> <p>My question is:</p> <p>Given a row vector of finite elements, what is the right way to calculate G(tau).</p> <p>The matlab code below shows two methods giving two different result. Which one is the correct one?</p> <pre><code>rawdata = [1 2 3 4 5 6 7 8]; %the regular method.Result shown in the next line %0.259259259 0.2 0.151515152 0.111111111 0.076923077 0.047619048 0.022222222 count = rawdata; Ntime = length(count); G = []; for t = 0:Ntime-1 top = []; bottom = []; ai = []; bi = []; ai = count(1:end-t); bi = count(t+1:end); top = mean( (ai-mean(ai)) .* (bi-mean(bi)) ); %bottom = mean(ai) * mean(bi); bottom = mean(count)^2; G = [G, top/bottom]; end %The typical FFT method. Results shown in the next line %0.259259259 0.086419753 -0.037037037 -0.111111111 -0.135802469 -0.111111111 -0.037037037 0.086419753 %NFFT euqals to the length of the input. count = rawdata; NFFT = 8; tmpGfft = length(count) * ifft( fft(count,NFFT).*conj(fft(count,NFFT)))/sum(count)^2 - 1; plot(G,'*');hold on; plot(tmpGfft,'o') </code></pre> <p>More reference on the technique itself, <a href="http://en.wikipedia.org/wiki/Fluorescence_correlation_spectroscopy" rel="nofollow">http://en.wikipedia.org/wiki/Fluorescence_correlation_spectroscopy</a></p>

http://asksci.com/questions/217/how-to-read-point-scan-lsm-files-in-matlab glucose 2010-04-02T16:50:28Z 2010-07-23T21:22:38Z

<p>How to read point scan lsm files in matlab</p>

http://asksci.com/questions/160/molecular-cloning-guide glucose 2010-03-04T22:22:36Z 2010-07-23T10:22:35Z

<p>Is there any good guide for doing the first molecular cloning experiment?</p>

http://asksci.com/questions/200/are-there-imagej-plugins-for-photon-counting-histogram-and-correlation-analysis glucose 2010-03-27T00:56:45Z 2010-07-20T11:22:36Z

<p>Are there ImageJ Plugins for Photon Counting Histogram and Correlation Analysis</p>

http://asksci.com/questions/237/what-is-hspg-in-biology glucose 2010-04-12T16:31:53Z 2010-07-19T21:22:35Z

<p>What is HSPG in biology?</p>

http://asksci.com/questions/166/how-to-do-in-vivo-imaging-with-mouse glucose 2010-03-10T02:07:57Z 2010-07-18T23:22:34Z

<p>I have read many papers about in vivo imaging with flies or fish. Is there any good set up to do in vivo imaging with mouse?</p>

http://asksci.com/questions/219/how-to-ask-for-a-postdoc-position glucose 2010-04-05T17:02:25Z 2010-07-18T23:22:34Z

<p>Can I just shoot a general email saying that I am interested and does your lab have any opening? Or ...</p>

http://asksci.com/questions/177/what-are-advantages-of-nanodrop glucose 2010-03-23T08:03:07Z 2010-07-18T22:13:07Z

<p>what are advantages of nanodrop</p>

http://asksci.com/questions/198/what-to-look-for-in-a-good-calibration-in-fcs glucose 2010-03-27T00:52:41Z 2010-07-17T23:22:36Z

<p>What to look for in a good Calibration in FCS</p>

http://asksci.com/questions/233/what-is-the-imagej-plugin-to-read-lsm-710-files glucose 2010-04-10T18:13:21Z 2010-07-17T23:22:36Z

<p>What is the imagej plugin to read lsm 710 files?</p>

http://asksci.com/questions/196/how-to-do-fcs-with-confocor glucose 2010-03-27T00:50:25Z 2010-07-17T04:22:36Z

<p>How to do FCS with confocor</p>

http://asksci.com/questions/186/how-to-do-imagej-live-dead-assay glucose 2010-03-26T05:35:23Z 2010-07-17T04:22:36Z

<p>How to do imagej live dead assay?</p>

http://asksci.com/questions/170/why-zeiss-lsm-710-is-the-perfect-confocoal-microscopy glucose 2010-03-18T22:28:39Z 2010-07-17T03:22:35Z

<p>We are trying to come up with reasons to justify buying the Zeiss LSM 710.</p> <p>What are the major selling points?</p>

http://asksci.com/questions/171/how-a-spinning-disc-confocal-works glucose 2010-03-22T18:28:45Z 2010-07-16T09:22:40Z

<p>how a spinning disc confocal works?</p>

http://asksci.com/questions/185/what-is-the-transfer-buffer-recipe glucose 2010-03-26T05:34:27Z 2010-07-16T08:22:36Z

<p>What is the transfer buffer recipe?</p> <p>sds 144 </p>

http://asksci.com/questions/4/what-is-systems-biology glucose 2009-10-07T21:29:21Z 2010-05-01T17:38:21Z

<p>Everyone is talking about systems biology nowadays. Is there any clear definition to it?</p>

http://asksci.com/questions/272/how-to-concentrate-protein-from-culture-medium glucose 2010-04-29T22:40:10Z 2010-04-29T22:40:10Z

<p>I want to concentrate protein in cell culture medium for western blot. </p> <p>Is there a simple method to do that?</p> <p>Any recommendation for columns?</p>

http://asksci.com/questions/278/circularly-polarized-lenses glucose 2010-05-06T17:08:01Z 2010-05-06T17:08:01Z

just try it. It doesn't hurt

http://asksci.com/questions/266/where-can-i-find-good-online-lectures-on-brain-evolution-for-an-introductory-cour/267#267 glucose 2010-04-29T16:47:57Z 2010-04-29T16:47:57Z

wow, that is not easy ...

http://asksci.com/questions/249/whats-the-scientific-term-for-borderline-significance-or-borderline-p-value/253#253 glucose 2010-04-19T05:32:37Z 2010-04-19T05:32:37Z

Thank you Kathe

http://asksci.com/questions/229/should-we-open-souce-our-lab-notebook/241#241 glucose 2010-04-17T07:14:37Z 2010-04-17T07:14:37Z

Maybe NIH can host the cloud, and do a pilot studies with some labs and see how it goes

http://asksci.com/questions/176/can-i-filter-solutions-containing-sds glucose 2010-03-26T06:20:16Z 2010-03-26T06:20:16Z

could not think of a reason why not

http://asksci.com/questions/182/mars-and-water glucose 2010-03-26T05:30:29Z 2010-03-26T05:30:29Z

It depends on the time of the day. I thought it should freeze immediately.

http://asksci.com/questions/159/what-is-a-pentane glucose 2010-03-05T08:15:37Z 2010-03-05T08:15:37Z

Pentane is an organic compound with the formula C 5 H 12

http://asksci.com/questions/145/primary-smooth-muscle-cell-culture glucose 2010-02-03T23:18:35Z 2010-02-03T23:18:35Z

hmmm not an expert in this topic ...

http://asksci.com/questions/85/no-bands-observed-in-western-blot-what-should-i-do/143#143 glucose 2010-02-02T18:35:57Z 2010-02-02T18:35:57Z

western blot is evil, isn't it?

http://asksci.com/questions/138/how-do-you-import-many-confocal-files-into-image-j/141#141 glucose 2010-02-01T19:16:10Z 2010-02-01T19:16:10Z

cool File_Opener is working for me thanks

http://asksci.com/questions/135/what-is-the-best-way-to-keep-a-record-of-confocal-experiments/136#136 glucose 2010-01-26T17:58:23Z 2010-01-26T17:58:23Z

nice... I was like , a lab notebook? ... Then...

http://asksci.com/questions/78/which-method-is-best-to-extract-dna-from-blood-sample/131#131 glucose 2010-01-22T19:32:07Z 2010-01-22T19:32:07Z

I think a kit is a safe bet

http://asksci.com/questions/75/glucose-comes-in-plz glucose 2009-10-26T23:56:47Z 2009-10-26T23:56:47Z

Email me at: admin at asksci.com.

http://asksci.com/questions/51/what-is-the-conservative-estimate-that-the-lhc-will-produce-a-dangerous-black-hol/74#74 glucose 2009-10-23T21:34:59Z 2009-10-23T21:34:59Z

Are you sure? I thought we are orbiting around sun because of its mass. If sun collapsed, meaning no mass, then how can earth still orbit around it?

http://asksci.com/questions/6/how-to-use-powerpoint-to-create-high-resolution-images-for-journal-publications glucose 2009-10-19T23:54:54Z 2009-10-19T23:54:54Z

no one in superuser.com cares about publication ...