I am trying to use fluorescence polarization to measure the size of complexes in serum and/or plasma. Does anyone know of a good fluor to use that will get around the problem of serum autofluorescence?
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First, can you measure the excitation and emission spectra for the serum you are interested in? Second, I assume you want to compare the serum spectra to the spectra of known probes, which can be found here: http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html |
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