Is there any good guide for doing the first molecular cloning experiment?

what happens if the environment around the tryptophan changes from Hydrophobic to hydrophilic ? I know that in fluorescence there is a red shift , but I don't know what happens i …

What is pcr and what is pcr used for?

I did some test to find out the function of a protein group. I would like to know of there are standard Test I can do, maybe something with a wider range of possibilities then what …

Hello, I`m currently working on the annotation of a (small) genome. This genome is very simular to that of an already annotated organism in the NCBI database, which implicates t …

moromou @ mitbbs.com Use linearized plasmid DNA(gel purified) as template, in vitro transcribe RNA probe, treat with DNase, run gel. Annoyingly, I got two bands, one is the right …

How to quantify DNA in solutions? What is the theory behind quantification?

Western blot is ...

Hello, I looked up SOE (splicing by overlap extension) in order to ligate two DNA fragments to each other with PCR. I fully understand the technique, although I`m still struggli …