I am currently redesigning some FRET probes and therefore I have the opportunity to optimize my FRET pair. Due to limited lasers available I am restricted to 488nm (EGFP) as donor …

what happens if the environment around the tryptophan changes from Hydrophobic to hydrophilic ? I know that in fluorescence there is a red shift , but I don't know what happens i …

Is there a complete database for flurohores?

There are lot of popular sources explaining how neutron stars are born, but I could not find any theory explaining death of neutron stars. What process make them burn ? Do they bur …

I am trying to use fluorescence polarization to measure the size of complexes in serum and/or plasma. Does anyone know of a good fluor to use that will get around the problem of s …

what is the T1/2 of GUS, GFP, RFP and YFP, RNA and proteins?

How much energy would be released for 3.5 moles of pentane. How much energy would be released for 10 moles of pentane. How much energy would be released for 0.75 moles of pentane.

I am trying to do some functional assays on primary smooth muscle cells from young mice. When I say young I mean newborn esophagus or 3 week old aortic smooth muscle. There are not …

Hello, I looked up SOE (splicing by overlap extension) in order to ligate two DNA fragments to each other with PCR. I fully understand the technique, although I`m still struggli …

Travel funds and time are limited, so perhaps someone here can give me a hint? I am aware of the International Conference On Comparative Cognition which usually features quite som …

Specifically, I'm interested in humidity and temperature, but weather, altitude, and any other conditions we are evolved for can be included. However, has there been enough time f …

roughly how many Kupffer Cells can you get from one liver? Or, does anyone know if there is a Kupffer cell line, or just Kupffer cell RNA commercially (or non-commercially) availa …

Do they still collaborate with each other on many projects? Just curious that Kolodkin's lab has not published anything last two years. I am interested in applying either lab for p …

Or how to calculate the diffusion coefficient based on the molecular weight?

Thinking about doing a post doc in his lab. Not sure he wants to take me, but is he nice? He is really smart, that is for sure...